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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Acute PDE4 Inhibition Induces a Transient Increase in Blood Glucose in Mice
doi: 10.3390/ijms24043260
Figure Lengend Snippet: PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by scintillation counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Article Snippet: After washing the columns twice with 1 mL of deionized water, the [ 3 H]-2-deoxyglucose-6-phosphate was eluted from the columns using 2 × 1 ml of 1.5 M NaCl, and the radioactivity in the eluates was then quantified using a
Techniques: Inhibition, Solvent, Control, Injection, Isolation, Chromatography, MANN-WHITNEY