scintillation liquid Search Results


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Revvity tri carb liquid scintillation counter
Tri Carb Liquid Scintillation Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity liquid scintillation counter
Liquid Scintillation Counter, supplied by Revvity, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Revvity tri carb 4910 tr
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Revvity liquid scintillation analyzer
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Liquid Scintillation Analyzer, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd cat 425 209 ecolite liquid scintillation cocktail mp biomedicals cat ic882475
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Cat 425 209 Ecolite Liquid Scintillation Cocktail Mp Biomedicals Cat Ic882475, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd cytoscint liquid scintillation cocktail
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Cytoscint Liquid Scintillation Cocktail, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Danaher Inc bcs na counting scintillation 150 cocktail
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Bcs Na Counting Scintillation 150 Cocktail, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd ecolume liquid scintillation cocktail
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Ecolume Liquid Scintillation Cocktail, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd betamax scintillation cocktail
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Betamax Scintillation Cocktail, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Valiant Co Ltd scintillation cocktail
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
Scintillation Cocktail, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chem Impex International maleimido ptsa para toluene sulfonic acid pdi polydispersity index phf poly
PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by <t>scintillation</t> counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).
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Image Search Results


PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by scintillation counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).

Journal: International Journal of Molecular Sciences

Article Title: Acute PDE4 Inhibition Induces a Transient Increase in Blood Glucose in Mice

doi: 10.3390/ijms24043260

Figure Lengend Snippet: PDE4 inhibition induces glycogenolysis in skeletal muscle and inhibits glucose uptake in skeletal muscle and heart. ( A ) PDE4 inhibition induces glycogenolysis in skeletal muscle. The glycogen levels in the liver and skeletal muscle (gastrocnemius) in postprandial mice are shown after treatment with the PAN-PDE4 inhibitor roflumilast (Rofl; 5 mg/kg; i.p.) or solvent control (Solv) for 90 min. Data are expressed as mg of glycogen per g of tissue weight. ( B ) PAN-PDE4 inhibition reduces locomotion, as reflected by reduced travel distance. Mice were injected with the PDE4 inhibitor rolipram (3 mg/kg; i.p.; n = 6) or solvent control, placed immediately in a new cage, and then locomotion was assessed using SmartCageTM technology. Traces represent changes in travel distance (cm per 5 min interval). ( C ) Postprandial mice were injected with the PDE4 inhibitor roflumilast (5 mg/kg; i.p.) or solvent control, followed 15 min later by injection with [ 3 H]-2-deoxyglucose (intravenous, i.v.). Animals were euthanized 30 min after the tracer injection, their tissues were homogenized, and the phosphorylated [ 3 H]-2-deoxyglucose-6-phosphate was isolated by anion exchange chromatography and quantified by scintillation counting. Data are expressed as pmol [ 3 H]-2-deoxyglucose-6-phosphate per g of tissue wet weight. All data represent the mean ± SEM. Statistical significance was determined using the Mann–Whitney test (bar graphs) or a two-way ANOVA with Sidak’s post hoc test (time courses) and is indicated as ns ( p > 0.05), * ( p < 0.05), and ** ( p < 0.01).

Article Snippet: After washing the columns twice with 1 mL of deionized water, the [ 3 H]-2-deoxyglucose-6-phosphate was eluted from the columns using 2 × 1 ml of 1.5 M NaCl, and the radioactivity in the eluates was then quantified using a liquid scintillation analyzer (PerkinElmer Tri-Carb ® 4810 TR).

Techniques: Inhibition, Solvent, Control, Injection, Isolation, Chromatography, MANN-WHITNEY